To plot the calibration curve, you need to prepare iron solutions with known concentrations and measure their absorbance.

You will obtain a standard iron solution containing[ 0.2500g/L] of pure iron. Pipet 25.00mL of this standard iron solution into a 500mL volumetric flask and dilute up to the mark with distilled water. Finally, you will pipet certain amounts of this diluted solution into different flasks.

How much of this diluted solution do you need to pipet to have 0.00mg of iron?

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